Technologies

BGene Genetics  is an innovative company specialised in metabolic and genetic engineering of bioproduced strains. In order to develop more environmentally friendly production ways, BGene engineers bacteria to perform a sustainable production of molecules from renewable and non-food raw materials using biotechnologies, computing science and microbial natural biodiversity.

 

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Colibelt®UNBELT®CAD4Bio

Characteristics

> Scarless

> Fully automated

> Achievable on a large number of bacteria

COLIBELT®

Creation of your bespoke bioproduction strains.

Undesirablle gene deletion, auxotrophy creation for antibiotic-free plasmids, promoter libraries, recombinant gene insertions in the chromosome, we analyse your needs and propose the best solution to your requirements. Firstly developped on E. coli, BGene technology allows to modify the bacterial genome precisely at one base.

Single gene or bioproduction pathway insertions, site directed mutagenesis or deletion are possible, without leaving any scar (repetitions, recombination site) nor antibiotic resistance. COLIBELT® may be utilised for any chromosomal modification on any E. coli gentoype. No particular feature of the strain is need.

This technology is fully automated.

Rapid, efficient, COLIBELT® allows to insert fragments as long as 10 kbps at a time in the chromosome, permitting to build new synthetic metabolic.

The modifications carried out to the strains are stable an no pressure selection is necessary to maintain the new strain features.

COLIBELT® can be applied as an alternative to plasmidic expression of recombinant proteins.

UNBELT®

A multi-purpose solution for bacterial chromosomal modifications

BGene technology is independent of genetic features, it is thus applicable to various bacteria. Stemming from COLIBELT® and assaye don both gram-negative and -positive strains, UNBELT® is the multi-purpose solution for bacterial chromosomal modification and allows to optimize industrial bacterial strains.

CAD4Bio®

The IT solution that ensures the automated platform follow-up dedicated to molecular biology.

Cad4Bio® comprises several features:

> Computer aided design for DNA Assembly. In silico design of DNA, in silico design and optimization of oligonucleotides, combinatory oriented. CAD4Bio® includes machine learning on assembly and sequencing result analysis.

> Workflow management for strain construction ensuring manual or high-throughput construction and traceability at any step.

> LIMS (Laboratory Information Management System) for strain DNA and oligo management. It also proposes a digital labbook.